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cell tracking fluorescent dyes  (Fisher Scientific)

 
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    Fisher Scientific cell tracking fluorescent dyes
    Cell Tracking Fluorescent Dyes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+tracking+fluorescent+dyes/cell+tracking+fluorescent+dyes/pmc06279597-53-19-26
    Average 90 stars, based on 1 article reviews
    cell tracking fluorescent dyes - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Tracking Assay:

    Article Title: Functional TCR T cell screening using single-cell droplet microfluidics
    Article Snippet: 7.5% sterile bovine serum albumin solution (BSA), 4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), 1H,1H,2H,2H-perfluoro-1-octanol, OPTIPREP and β-mercaptoethanol were purchased from Sigma-aldrich. .. RPMI 1640, IMDM (GlutaMAX Supplement), MEM Non-Essential Amino Acids (NEAA), sodium pyruvate, 200g/L glucose solution, Penicillin Streptomycin solution and cell tracking fluorescent dyes were purchased from Fisher Scientific. ..

    Article Title: Functional TCR T cell screening using single-cell droplet microfluidics
    Article Snippet: .. D ow nl oa de d by T ul an e U ni ve rs ity o n 1/ 21 /2 01 9 8: 03 :5 7 A M Lab Chip, 2018, 18, 3733–3749 | 3735This journal is © The Royal Society of Chemistry 2018 RPMI 1640, IMDM (GlutaMAX supplement), MEM nonessential amino acids (NEAA), sodium pyruvate, 200 g L−1 glucose solution, penicillin streptomycin solution and cell tracking fluorescent dyes were purchased from Fisher Scientific. ..



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    MEG-01-derived IL-8 promotes proliferation and invasion of prostate cancer cells. (A, B) MEG-01 cells were pre-labeled with <t>CMTPX,</t> a red <t>fluorescent</t> cell tracker prior to their placement in 1 μm pore-sized insert. The insert was mounted on the well with cancer cells (LNCaP or PC-3) and incubated in serum-starved (1%) condition for 24 h. The migrated microparticles of MEG-01 cells were visualized using fluorescence microscopy at 200× magnification (A). The number of cancer cells in the lower well was counted (B). The bar graphs represent the means ± SEM from three independent experiments. * p <0.05, compared to LNCaP cells without co-culture. # p <0.05, compared to MEG-01-untreated group in each cell line. (C) Invasion assay of LNCaP and PC-3 in the absence or presence of MEG-01 cells. Cancer cells were seeded in an 8 μm pore-sized insert and mounted on a well that lacked or contained MEG-01 cells, and incubated for 24 h. The invading cancer cells were stained, photographed, and counted (n=3). * p <0.05, compared to LNCaP cell without co-culture. # p <0.05 compared to MEG-01-untreated group in each cell line. (D) Invasion assay of IL-8-silenced PC-3 cells in the absence and presence of MEG-01 cells for 24 h (n=3). * p <0.05, compared to siNT. # p <0.05, compared to si IL-8 -treated group. (E, F) CXCL chemokine levels were measured by ELISA (n=3). CXCL chemokines in culture supernatant of MEG-01 cells without cancer cells (E) and with cancer cell co-culture (F). Culture conditions were the same as described in (A). * p <0.05, compared to MEG-01-untreated group.
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    MEG-01-derived IL-8 promotes proliferation and invasion of prostate cancer cells. (A, B) MEG-01 cells were pre-labeled with <t>CMTPX,</t> a red <t>fluorescent</t> cell tracker prior to their placement in 1 μm pore-sized insert. The insert was mounted on the well with cancer cells (LNCaP or PC-3) and incubated in serum-starved (1%) condition for 24 h. The migrated microparticles of MEG-01 cells were visualized using fluorescence microscopy at 200× magnification (A). The number of cancer cells in the lower well was counted (B). The bar graphs represent the means ± SEM from three independent experiments. * p <0.05, compared to LNCaP cells without co-culture. # p <0.05, compared to MEG-01-untreated group in each cell line. (C) Invasion assay of LNCaP and PC-3 in the absence or presence of MEG-01 cells. Cancer cells were seeded in an 8 μm pore-sized insert and mounted on a well that lacked or contained MEG-01 cells, and incubated for 24 h. The invading cancer cells were stained, photographed, and counted (n=3). * p <0.05, compared to LNCaP cell without co-culture. # p <0.05 compared to MEG-01-untreated group in each cell line. (D) Invasion assay of IL-8-silenced PC-3 cells in the absence and presence of MEG-01 cells for 24 h (n=3). * p <0.05, compared to siNT. # p <0.05, compared to si IL-8 -treated group. (E, F) CXCL chemokine levels were measured by ELISA (n=3). CXCL chemokines in culture supernatant of MEG-01 cells without cancer cells (E) and with cancer cell co-culture (F). Culture conditions were the same as described in (A). * p <0.05, compared to MEG-01-untreated group.
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    MEG-01-derived IL-8 promotes proliferation and invasion of prostate cancer cells. (A, B) MEG-01 cells were pre-labeled with <t>CMTPX,</t> a red <t>fluorescent</t> cell tracker prior to their placement in 1 μm pore-sized insert. The insert was mounted on the well with cancer cells (LNCaP or PC-3) and incubated in serum-starved (1%) condition for 24 h. The migrated microparticles of MEG-01 cells were visualized using fluorescence microscopy at 200× magnification (A). The number of cancer cells in the lower well was counted (B). The bar graphs represent the means ± SEM from three independent experiments. * p <0.05, compared to LNCaP cells without co-culture. # p <0.05, compared to MEG-01-untreated group in each cell line. (C) Invasion assay of LNCaP and PC-3 in the absence or presence of MEG-01 cells. Cancer cells were seeded in an 8 μm pore-sized insert and mounted on a well that lacked or contained MEG-01 cells, and incubated for 24 h. The invading cancer cells were stained, photographed, and counted (n=3). * p <0.05, compared to LNCaP cell without co-culture. # p <0.05 compared to MEG-01-untreated group in each cell line. (D) Invasion assay of IL-8-silenced PC-3 cells in the absence and presence of MEG-01 cells for 24 h (n=3). * p <0.05, compared to siNT. # p <0.05, compared to si IL-8 -treated group. (E, F) CXCL chemokine levels were measured by ELISA (n=3). CXCL chemokines in culture supernatant of MEG-01 cells without cancer cells (E) and with cancer cell co-culture (F). Culture conditions were the same as described in (A). * p <0.05, compared to MEG-01-untreated group.
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    Image Search Results


    MEG-01-derived IL-8 promotes proliferation and invasion of prostate cancer cells. (A, B) MEG-01 cells were pre-labeled with CMTPX, a red fluorescent cell tracker prior to their placement in 1 μm pore-sized insert. The insert was mounted on the well with cancer cells (LNCaP or PC-3) and incubated in serum-starved (1%) condition for 24 h. The migrated microparticles of MEG-01 cells were visualized using fluorescence microscopy at 200× magnification (A). The number of cancer cells in the lower well was counted (B). The bar graphs represent the means ± SEM from three independent experiments. * p <0.05, compared to LNCaP cells without co-culture. # p <0.05, compared to MEG-01-untreated group in each cell line. (C) Invasion assay of LNCaP and PC-3 in the absence or presence of MEG-01 cells. Cancer cells were seeded in an 8 μm pore-sized insert and mounted on a well that lacked or contained MEG-01 cells, and incubated for 24 h. The invading cancer cells were stained, photographed, and counted (n=3). * p <0.05, compared to LNCaP cell without co-culture. # p <0.05 compared to MEG-01-untreated group in each cell line. (D) Invasion assay of IL-8-silenced PC-3 cells in the absence and presence of MEG-01 cells for 24 h (n=3). * p <0.05, compared to siNT. # p <0.05, compared to si IL-8 -treated group. (E, F) CXCL chemokine levels were measured by ELISA (n=3). CXCL chemokines in culture supernatant of MEG-01 cells without cancer cells (E) and with cancer cell co-culture (F). Culture conditions were the same as described in (A). * p <0.05, compared to MEG-01-untreated group.

    Journal: Biomolecules & Therapeutics

    Article Title: Megakaryocyte-Derived IL-8 Acts as a Paracrine Factor for Prostate Cancer Aggressiveness through CXCR2 Activation and Antagonistic AR Downregulation

    doi: 10.4062/biomolther.2023.005

    Figure Lengend Snippet: MEG-01-derived IL-8 promotes proliferation and invasion of prostate cancer cells. (A, B) MEG-01 cells were pre-labeled with CMTPX, a red fluorescent cell tracker prior to their placement in 1 μm pore-sized insert. The insert was mounted on the well with cancer cells (LNCaP or PC-3) and incubated in serum-starved (1%) condition for 24 h. The migrated microparticles of MEG-01 cells were visualized using fluorescence microscopy at 200× magnification (A). The number of cancer cells in the lower well was counted (B). The bar graphs represent the means ± SEM from three independent experiments. * p <0.05, compared to LNCaP cells without co-culture. # p <0.05, compared to MEG-01-untreated group in each cell line. (C) Invasion assay of LNCaP and PC-3 in the absence or presence of MEG-01 cells. Cancer cells were seeded in an 8 μm pore-sized insert and mounted on a well that lacked or contained MEG-01 cells, and incubated for 24 h. The invading cancer cells were stained, photographed, and counted (n=3). * p <0.05, compared to LNCaP cell without co-culture. # p <0.05 compared to MEG-01-untreated group in each cell line. (D) Invasion assay of IL-8-silenced PC-3 cells in the absence and presence of MEG-01 cells for 24 h (n=3). * p <0.05, compared to siNT. # p <0.05, compared to si IL-8 -treated group. (E, F) CXCL chemokine levels were measured by ELISA (n=3). CXCL chemokines in culture supernatant of MEG-01 cells without cancer cells (E) and with cancer cell co-culture (F). Culture conditions were the same as described in (A). * p <0.05, compared to MEG-01-untreated group.

    Article Snippet: For visualization of diffused MEG-01-derived microparticles, MEG-01 cells were stained with 5 μM of the cell-tracking red fluorescent dye CMTPX (Thermo Fisher Scientific).

    Techniques: Derivative Assay, Labeling, Incubation, Fluorescence, Microscopy, Co-Culture Assay, Invasion Assay, Staining, Enzyme-linked Immunosorbent Assay